Dissecting Hematopoietic stem cell fate using Ki67-RFP knock-in mouse line


Congrats to Tatyana Grinenko and Ben Wielockx from Dresden, Germany! The collaboration we initiated around 2 years ago, using the Ki67-RFP mouse line to identify and purify dividing blood stem cells, is now a part of the recent Nature Communications article “Hematopoietic stem cells can differentiate into restricted myeloid progenitors before cell division in mice” (https://rdcu.be/ONi2).

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Seminar at BCRM

Onur Basak will give a seminar on the 22nd of March 2018 at the David de Wied room on the 4th floor of Stratenum, at 9. The talk will be an introduction to of the past and present projects to the Translational Neuroscience department, and is titled “Modelling neural stem cell dynamics in space and time”



PNAS publication on adult neural stem cell dynamics

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Adult mammalian tissues contain stem cells that contribute to tissue homeostasis and regeneration, with potential therapeutic applications. Specialized niches regulate their fate. Here we evaluated quantitatively how the subependymal zone niche regulates neural stem cell (NSC) number in the adult mouse brain. Using knock-in reporter alleles and single-cell RNA sequencing, we show that the Wnt target Tnfrsf19/Troy identifies both active and quiescent NSCs. Using the Ki67- iresCreER mouse model, we found that dividing stem cells have long-term self-renewal potential. We propose a model where the fate of NSCs is coupled to their density within a closed niche. Our results suggest a new mechanism for regulating adult stem cell number, which might be deregulated in brain malignancies and in aging.

link: http://www.pnas.org/content/early/2018/01/05/1715911114.full